28/11/2020
Fluorometer technical background
Our nano series Micro-Spectrophotometer measure the concentration and purity of the sample, because it is able to measure the absorbance of the whole ingredient(such as DNA, RNA, oligos and so on) of the sample at 260nm, that may make the read inaccuracy. While the fluorometer measure the fluorescence intensity of the fluorescent reagent which combine with the target molecule to read the concentration of sample. Generally, the read is lower than Nano series products but much accuracy than it
The sensitivity of Nano series products are above 5ng/ul, and while the fluorometer is 0.5pg/ul in measuring DNA concentration. It is important and necessary for the most applications to read the lower and accuracy concentration of the sample. Using the Fluo-100 fluorometer with the quantitation kit can measure the sample concentration rapidly, sensitively and accurately
Fluo-100 Simple, easy to use and applications
The Fluo-100 is a dual-channel fluorometer, it provides high-sensitive fluorescent detection when quantifying nucleic acids and proteins, simple and cost to operate
Applications:
1. The sample is scarce and difficult in processing
2. The low quantity of DNA, RNA, or protein after extraction
3. The sample will be used in the expensive experiments: qPCR, PCR cloning, transfection, the next generation sequencing and etc.
Two detection models of Fluo-100
Code Light Excitation Filters Emission Filters Fluo-100A UV 365±20nm 420-480nm (60nm) Blue 460±20nm 525-570nm (45nm) Fluo-100B Blue 460±20nm 525-570nm (45nm) Red 625±10nm 670-725nm (55nm) Fluo-100C Blue 460±20nm 525-570nm (45nm) Green 525±20nm 575-640nm (65nm)
Fluo-100 Quantitation kit
High sensitivity of dsDNA Quantitation Kit: 0.2--100ng
High sensitivity of ssDNA Quantitation Kit: 1--200ng
Broad range of dsDNA Quantitation Kit: 100--1600ng
Protein Quantitation Kit is researching
Fluo-100 Quantitation kit operation

Application of different fluorescence channel
ChannelExcitation Filters Normal kits ApplicationsUV channel 365±20nm Hoechst 33258, 4-MU,
EnZCheK Caspase Nuclear acid quantification, Plant GUS reporter gene detection, Poptosis
